Includes cutting-edge methods and protopls

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

In Vivo Models of Inflammatory Bowel Disease and Colitis-Associated Cancer.- Combining Cell Type-Restricted Adenoviral Targeting with Immunostaining and Flow Cytometry to Identify Cells-of-Origin of Lung Cancer.- Utility of Endoscopic Ultrasound-Guided Fine-Needle Aspiration for Preclinical Evaluation of Therapies in Cancer.- Modelling Intestinal Carcinogenesis Using In Vitro OrganoidCultures.- Protocols to Evaluate Cigarette Smoke-induced Lung Inflammationand Pathology in Mice.- Tracking Competent Host Defence to Chronic Inflammation: An In vivo Model of Peritonitis.- Citrobacter rodentium Infection Model for the Analysis of Bacterial Pathogenesis and Mucosal Immunology.- In Vivo Infection Model of Severe Influenza a Virus.- In Vivo Models for Inflammatory Arthritis.- Isolation of Mouse Primary Gastric Epithelial Cells to Investigate the Mechanisms of Helicobacter pylori Associated Disease.- Dissecting lnterleukin-6 Classic- and Trans-Signaling in Inflammation and Cancer.- Laser Microdissection of Cellular Compartments for Expression Analyses in Cancer Models.- Tissue Processing for Stereological Analyses of Lung Structure in Chronic Obstructive Pulmonary Disease.- Quantifying Caspase-1 Activity in Murine Macrophages.- Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation.- Analysis of DNA Methylation in Tissues Exposed to Inflammation.- A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens.- Immuno-detection of Immature and Bioactive Forms of the Inflammatory Cytokine IL-18.- Optimisation Techniques for miRNA Expression in Low Frequency Immune Cell Populations.- Assessing the cGAS-cGAMP-STING Activity of Cancer Cells.- Expression and Purification of JAK1 and SOCS1 for Structural and Biochemical Studies.- Production of Recombinant Killer Immunoglobulin-like Receptors for Crystallography and Luminex Based Assays.- Isolation and Characterisation of Mouse Intrahepatic Lymphocytes by Flow Cytometry.