Jr McCoy: Immunophenotyping | Buch

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Cover: 9781493996490 | Immunophenotyping | Methods and Protocols | Jr McCoy | Buch | X | 2019

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Sprache: Englisch

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Beschreibung

This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolvingfield.

Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis

High Dimensional Immunophenotyping with Fluorescence-based Cytometry: A Practical Guidebook.- Immunophenotyping by Mass Cytometry.- Fluorescent Cell Barcoding for Immunophenotyping.- Immunophenotyping using Dried and Lyophilized Reagents.- Guidelines for Gating Flow Cytometry Data for Immunological Assays.- Strategies and Techniques for NK Cell Phenotyping.- Immunophenotyping of Human B Lymphocytes in the Blood and in the Adipose Tissue.- Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T Cells.- Immunophenotyping of Human Regulatory T Cells.- Immunophenotyping of Human Innate Lymphoid Cells.- Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis.- Immunophenotyping of Circulating Endothelial Cell and Endothelial Microparticles.- Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells.- Quality Control of Immunophenotyping.- Immunophenotyping of Acute Myeloid Leukemia.- Immunophenotyping of Acute Lymphoblastic Leukemia.- Clinical Flow Cytometry Testing in Chronic Lymphocytic Leukemia.- Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH).

Details

Erscheinungsjahr:	2019
Fachbereich:	Therapie
Genre:	Medizin
Rubrik:	Wissenschaften
Medium:	Buch
Seiten:	368
Reihe:	Methods in Molecular Biology
Inhalt:	x 356 S. 15 s/w Illustr. 79 farbige Illustr. 356 p. 94 illus. 79 illus. in color.
ISBN-13:	9781493996490
ISBN-10:	1493996495
Sprache:	Englisch
Ausstattung / Beilage:	HC runder Rücken kaschiert
Einband:	Gebunden
Redaktion:	McCoy, Jr
Herausgeber:	J Philip McCoy Jr
Auflage:	1st ed. 2019
Hersteller:	Springer New York Springer US, New York, N.Y. Methods in Molecular Biology
Maße:	260 x 183 x 26 mm
Von/Mit:	Jr McCoy
Erscheinungsdatum:	15.09.2019
Gewicht:	0,889 kg