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Immunoassays
A Practical Approach
Taschenbuch von James P. Gosling
Sprache: Englisch

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Beschreibung
Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for
preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to
combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give
valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.
Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for
preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to
combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give
valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.
Inhaltsverzeichnis
  • Analysis by specific binding

  • Raising antibodies

  • Choosing and characterizing antibodies

  • Labels and endpoints

  • Solid-phase reagents

  • Standard and immunogens

  • Optimization

  • Validation

  • Data processing

  • Quality assurance

Details
Erscheinungsjahr: 2000
Fachbereich: Pharmazie
Genre: Medizin
Rubrik: Wissenschaften
Medium: Taschenbuch
ISBN-13: 9780199637102
ISBN-10: 0199637105
Sprache: Englisch
Ausstattung / Beilage: Paperback
Einband: Kartoniert / Broschiert
Redaktion: Gosling, James P.
Hersteller: OUP Oxford
Maße: 246 x 189 x 18 mm
Von/Mit: James P. Gosling
Erscheinungsdatum: 15.06.2000
Gewicht: 0,637 kg
Artikel-ID: 108635605
Inhaltsverzeichnis
  • Analysis by specific binding

  • Raising antibodies

  • Choosing and characterizing antibodies

  • Labels and endpoints

  • Solid-phase reagents

  • Standard and immunogens

  • Optimization

  • Validation

  • Data processing

  • Quality assurance

Details
Erscheinungsjahr: 2000
Fachbereich: Pharmazie
Genre: Medizin
Rubrik: Wissenschaften
Medium: Taschenbuch
ISBN-13: 9780199637102
ISBN-10: 0199637105
Sprache: Englisch
Ausstattung / Beilage: Paperback
Einband: Kartoniert / Broschiert
Redaktion: Gosling, James P.
Hersteller: OUP Oxford
Maße: 246 x 189 x 18 mm
Von/Mit: James P. Gosling
Erscheinungsdatum: 15.06.2000
Gewicht: 0,637 kg
Artikel-ID: 108635605
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