Dr. YURI KAZAKEVICH is an Associate Professor of Analytical Chemistry at Seton Hall University with thirty years of experience in liquid chromatography. He has over fifteen years of academic research experience in Russia and fifteen years of research, teaching and consulting for major American pharmaceutical companies, which has resulted in numerous papers and four book chapters. The main focus of Dr. Kazakevich's research is in HPLC retention mechanisms and the description of the analyte migration through the column with an emphasis on the influence of adsorbent geometry and surface chemistry. Dr. Kazakevich is the author of Basic Liquid Chromatography, one of the oldest and most complete online instructional resources on HPLC.
Dr. ROSARIO LOBRUTTO has over thirteen years of experience in the pharmaceutical industry working both with drug substances and drug products. He is a Group Head in the Pharmaceutical and Analytical Development Department at Novartis Pharmaceuticals Corporation managing multiple early to late phase projects for both small drug molecules and proteins/peptides and is responsible for teaching in-house HPLC method development training. Dr. LoBrutto is the author of twenty research articles, fifty presentations, and five book chapters in different areas of analytical/physical chemistry and has served as an adjunct professor for seven years. Dr. LoBrutto's main research directions include HPLC retention mechanisms, the influence of mobile phase modifiers on analyte retention and chromatographic figures of merit, and method development strategies for small molecules and proteins/peptides.

PREFACE.

CONTRIBUTORS.

PART I HPLC THEORY AND PRACTICE.

1 Introduction (Yuri Kazakevich and Rosario LoBrutto).

2 HPLC Theory (Yuri Kazakevich).

3 Stationary Phases (Yuri Kazakevich and Rosario LoBrutto).

4 Reversed-Phase HPLC (Rosario LoBrutto and Yuri Kazakevich).

5 Normal-Phase HPLC (Yong Liu and Anant Vailaya).

6 Size-Exclusion Chromatography (Yuri Kazakevich and Rosario LoBrutto).

7 LC/MS: Theory, Instrumentation, and Applications to Small Molecules (Guodong Chen, Li-Kang Zhang, and Birendra N. Pramanik).

8 Method Development (Rosario LoBrutto).

9 Method Validation (Rosario LoBrutto and Tarun Patel).

10 Computer-Assisted HPLC and Knowledge Management (Yuri Kazakevich, Michael McBrien, and Rosario LoBrutto).

PART II HPLC IN THE PHARMACEUTICAL INDUSTRY.

11 The Expanding Role of HPLC in Drug Discovery (Daniel B. Kassel).

12 Role of HPLC in Preformulation (Irina Kazakevich).

13 The Role of Liquid Chromatography-Mass Spectrometry in Pharmacokinetics and Drug Metabolism (Ray Bakhtiar, Tapan K. Majumdar, and Francis L. S. Tse).

14 Role of HPLC in Process Development (Richard Thompson and Rosario LoBrutto).

15 Role of HPLC During Formulation Development (Tarun S. Patel and Rosario LoBrutto).

16 The Role of HPLC in Technical Transfer and Manufacturing (Joseph Etse).

PART III HYPHENATED TECHNIQUES AND SPECIALIZED HPLC SEPARATIONS.

17 Development of Fast HPLC Methods (Anton D. Jerkovich and Richard V. Vivilecchia).

18 Temperature as a Variable in Pharmaceutical Applications (Roger M. Smith).

19 LC/MS Analysis of Proteins and Peptides in Drug Discovery (Guodong Chen, Yan-Hui Liu, and Birendra N. Pramanik).

20 LC-NMR Overview and Pharmaceutical Applications (Maria Victoria Silva Elipe).

21 Trends in Preparative HPLC (Ernst Kuesters).

22 Chiral Separations (Nelu Grinberg, Thomas Burakowski, and Apryll M. Stalcup).

CHEMICAL AND DRUG COMPOUND INDEX.

SUBJECT INDEX.