Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Preface...Table of Contents...Contributing Authors...
1. Challenges in Developing Protein Secretion Assays at a Single-Cell LevelYoshitaka Shirasaki and Osamu Ohara
2. To Count of Not to Count: ELISPOT Evaluation RevisitedSylvia Janetzki
3. Essential Controls for ELISpot AssayJodi Hagen and Alex Kalyuzhny
4. Automatic Search of Spots and Color Classification in ELISPOT AssaySergey S. Zadorozhny and Nikolai N. Martynov
5. Four Color ImmunoSpot ® Assays for Identification of Effector T Cell LineagesJodi Hanson, Diana R. Roen, and Paul V. Lehmann
6. Detection of Cross-Reactive B Cells Using the FluoroSpot Assay Peter Jahnmatz and Niklas Ahlborg
7. Multiplex ImmunoSpot® Assays for the Study of Functional B Cell SubpopulationsDiana R. Roen, Jodi Hanson, and Paul V. Lehmann
8. Detecting All Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® AssaysRichard Caspell and Paul V. Lehmann
9. Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance AlgorithmAlexey Y. Karulin, Zoltán Megyesi, Richard Caspell, Jodi Hanson, and Paul V. Lehmann
10. Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X(TM) SuiteZoltán Megyesi, Paul V. Lehmann, and Alexey Y. Karulin
11. B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells Haw Hwai, Yi-Ying Chen, and Siang-Jong Tzeng
12. Identification of Novel Mycobacterial Targets for Murine CD4+T Cells by IFN ELISPOTAlison J. Johnson, Steven C. Kennedy, Tony W. Ng, and Steven A. Porcelli
13. ELISPOT-Based "Multi-Color FluoroSpot" to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus InfectionsPaulina Andrade, Josefina Coloma, and Eva Harris
14. Cultured ELISpot Assay to Investigate Dengue Virus Specific T Cell ResponsesChandima Jeewandara, Graham S. Ogg, and Gathsaurie Neelika Malavige
15. Ex Vivo ELISpot Assay to Investigate Dengue Virus Specific T Cell ResponsesGathsaurie Neelika Malavige
16. Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue InfectionAchala Indika Kamalasasa and Gathsaurie Neelika Malavige
17. Dendritic Cell-Based ELISpot Assay for Assessing T Cell-IFN- ¿ Responses in Human Peripheral Blood Mononuclear Cells to Dengue Envelope ProteinsPeifang Sun and Monika Simmons
18. Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP VaccinesBikash Sahay, Alek M. Aranyos, Andrew McAvoy, and Janet K. Yamamoto
19. Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs using ELISpot AssayIana H. Haralambieva, Inna G. Ovsyannikova, Richard B. Kennedy, and Gregory A. Poland