Features protocols in glyco-engineering in bacteria, yeast, insects, plants and mammalian cells

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Current Approaches to Engineering N-Linked Protein Glycosylation in Bacteria.- Inverse Metabolic Engineering for Enhanced Glycoprotein Production in Escherichia coli.- GlycoSNAP: A High-Throughput Screening Methodology for Engineering Designer Glycosylation Enzymes.- Production of Glycoproteins with Asparagine-Linked N-Acetylglucosamine in Escherichia coli.- Glyco-Engineering O-Antigene-Based Vaccines and Diagnostics in E. coli.- Progress in Yeast Glycosylation Engineering.- Protein Production with a Pichia pastoris OCH1 Knock-Out Strain in Fed-Batch Mode.- Engineering the Pichia pastoris N-Glycosylation Pathway Using the GlycoSwitch Technology.- Development of a Valuable Yeast Strain Using a Novel Mutagenesis Technique for the Effective Production of Therapeutic Glycoproteins.- An Overview and History of Glyco-Engineering in Insect Expression Systems.- SweetBac: Applying MultiBac Technology Towards Flexible Modification of Insect Cell Glycosylation.- Engineering Sialic Acid Synthesis Ability in Insect Cells.- Engineering N-Glycosylation Pathway in Insect Cells: Suppression of ß-N-Acetylglucosaminidase and Expression of ß-1,4-Galactosyltransferase.- N-Glyco-Engineering in Plants: Update on Strategies and Major Achievements.- Gene Targeting for Precision Glyco-Engineering: Production of Biopharmaceuticals Devoid of Plant-Typical Glycosylation in Moss Bioreactors.- Im"plant"ing of Mammalian Glycosyltransferase Gene into Plant Suspension-Cultured Cells Using Agrobacterium-Mediated Transformation.- Transient Glyco-Engineering of N. benthamianaAiming at the Synthesis of Multi-Antennary Sialylated Proteins.- Subcellular Targeting of Proteins Involved in Modification of plant N- and O-Glycosylation.- Assembly of Multigene Constructs Using Golden Gate Cloning.- Strategies for Engineering Protein N-Glycosylation Pathway in Mammalian Cells.- Glycan Remodelling with Processing Inhibitors and Lectin-Resistant