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Fundamentals of Fluorescence Microscopy
Exploring Life with Light
Buch von Alberto Diaspro (u. a.)
Sprache: Englisch

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Beschreibung
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors.
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors.
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
Über den Autor

Alberto Diaspro, born in Genoa, Italy, on April 7, 1959, received his Laurea in Electronic Engineering in 1983, [...] Genoa. Dir. Nanophys at Italian Institute of Technology (IIT), Deputy Director of IIT, Prof. Appl. Phys. at [...] Genoa. President of Optics Within Life Sciences, IEEE Senior member and Editor in chief of Microscopy Research and Technique (7/2013).
> 250, H=30, cit > 4000 (Google Scholar). AD isinvolved in research development of instrumentation for applications in biophysics and biomedical engineering and in nanobiotechnological projects.

Partha Pratim Mondal is an assistant professor at the Indian Institute of Science in the department of Instrumentation and Applied Physics. He received his Ph.D. in Physics from the Indian Institute of Science, Bangalore, India in 2005. He has been a researcher at the MIT, Cambridge, MA, USA and at the universities of Genova, Italy and also was a ICTP Fellow, Trieste, Italy. He has more than 30 journal publications to his name and 18 contributions to conference proceedings. Currently, he is Associate Editor for Scientific Reports (Nature Publishing) , Academic Editor to the AIP Advances journal and an editorial board member to 'Microscopy, Research and Technique (Wiley).

Zusammenfassung

A comprehensive text that starts from the fundamental optical imaging concepts

Guides the readers to the most advanced methods in fluorescence imaging and super-resolution techniques

An essential text for courses in imaging optics, photophysics, fluorescence microscopy and imaging, methods in cell biology

A thorough introduction to fluorescence microscopy and imaging for students in biophysics, molecular and cell biology, biomedical engineering, applied physics

Contains three useful Appendices

Lists of recommended books at the end of each chapter, to expand on various specialized topics

Written by two acknowledged experts in this rapidly evolving, interdisciplinary field

Includes supplementary material: [...]

Inhaltsverzeichnis
Preface.- Acknowledgements.- Dedication.- Ray Optics, Wave Optics and Imaging System Designing.- Basics of Electromagnetic Theory for Fluorescence Microscopy.- Electric Field Effects in Optical Microscopy Systems.- Quantum Description of Radiation Field and Optical Microscopy.- Molecular Physics of Fluorescence Markers.- Basics of Fluorescence and Photophysics.- General Fluorescence Imaging Techniques.- Multiphoton Fluorescence Microscopy.- Super Resolution Fluorescence Microscopy.- Image Reconstruction Methodologies for Fluorescence Microscopy.- Future Prospective of Fluorescence Microscopy.- Appendix I.- Appendix II.- Appendix III.
Details
Erscheinungsjahr: 2014
Fachbereich: Mechanik & Akustik
Genre: Physik
Rubrik: Naturwissenschaften & Technik
Medium: Buch
Inhalt: xv
218 S.
85 s/w Illustr.
55 farbige Illustr.
218 p. 140 illus.
55 illus. in color.
ISBN-13: 9789400775442
ISBN-10: 940077544X
Sprache: Englisch
Herstellernummer: 86061629
Ausstattung / Beilage: HC runder Rücken kaschiert
Einband: Gebunden
Autor: Diaspro, Alberto
Mondal, Partha Pratim
Hersteller: Springer Netherland
Springer Netherlands
Maße: 285 x 215 x 19 mm
Von/Mit: Alberto Diaspro (u. a.)
Erscheinungsdatum: 08.01.2014
Gewicht: 0,84 kg
Artikel-ID: 105694827
Über den Autor

Alberto Diaspro, born in Genoa, Italy, on April 7, 1959, received his Laurea in Electronic Engineering in 1983, [...] Genoa. Dir. Nanophys at Italian Institute of Technology (IIT), Deputy Director of IIT, Prof. Appl. Phys. at [...] Genoa. President of Optics Within Life Sciences, IEEE Senior member and Editor in chief of Microscopy Research and Technique (7/2013).
> 250, H=30, cit > 4000 (Google Scholar). AD isinvolved in research development of instrumentation for applications in biophysics and biomedical engineering and in nanobiotechnological projects.

Partha Pratim Mondal is an assistant professor at the Indian Institute of Science in the department of Instrumentation and Applied Physics. He received his Ph.D. in Physics from the Indian Institute of Science, Bangalore, India in 2005. He has been a researcher at the MIT, Cambridge, MA, USA and at the universities of Genova, Italy and also was a ICTP Fellow, Trieste, Italy. He has more than 30 journal publications to his name and 18 contributions to conference proceedings. Currently, he is Associate Editor for Scientific Reports (Nature Publishing) , Academic Editor to the AIP Advances journal and an editorial board member to 'Microscopy, Research and Technique (Wiley).

Zusammenfassung

A comprehensive text that starts from the fundamental optical imaging concepts

Guides the readers to the most advanced methods in fluorescence imaging and super-resolution techniques

An essential text for courses in imaging optics, photophysics, fluorescence microscopy and imaging, methods in cell biology

A thorough introduction to fluorescence microscopy and imaging for students in biophysics, molecular and cell biology, biomedical engineering, applied physics

Contains three useful Appendices

Lists of recommended books at the end of each chapter, to expand on various specialized topics

Written by two acknowledged experts in this rapidly evolving, interdisciplinary field

Includes supplementary material: [...]

Inhaltsverzeichnis
Preface.- Acknowledgements.- Dedication.- Ray Optics, Wave Optics and Imaging System Designing.- Basics of Electromagnetic Theory for Fluorescence Microscopy.- Electric Field Effects in Optical Microscopy Systems.- Quantum Description of Radiation Field and Optical Microscopy.- Molecular Physics of Fluorescence Markers.- Basics of Fluorescence and Photophysics.- General Fluorescence Imaging Techniques.- Multiphoton Fluorescence Microscopy.- Super Resolution Fluorescence Microscopy.- Image Reconstruction Methodologies for Fluorescence Microscopy.- Future Prospective of Fluorescence Microscopy.- Appendix I.- Appendix II.- Appendix III.
Details
Erscheinungsjahr: 2014
Fachbereich: Mechanik & Akustik
Genre: Physik
Rubrik: Naturwissenschaften & Technik
Medium: Buch
Inhalt: xv
218 S.
85 s/w Illustr.
55 farbige Illustr.
218 p. 140 illus.
55 illus. in color.
ISBN-13: 9789400775442
ISBN-10: 940077544X
Sprache: Englisch
Herstellernummer: 86061629
Ausstattung / Beilage: HC runder Rücken kaschiert
Einband: Gebunden
Autor: Diaspro, Alberto
Mondal, Partha Pratim
Hersteller: Springer Netherland
Springer Netherlands
Maße: 285 x 215 x 19 mm
Von/Mit: Alberto Diaspro (u. a.)
Erscheinungsdatum: 08.01.2014
Gewicht: 0,84 kg
Artikel-ID: 105694827
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