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Beschreibung
Flow cytometry (FCM) is the most practiced member of a family of technologies known variously as automated, analytical and quantitative cytology. Flow cytometry DNA content analysis of human neoplasia provides quantitative information on DNA ploidy, clonal DNA heterogeneity and proliferative activity. The wide use of FCM also stems from the fact that this methodology provides a mean to study the properties of large cell populations rather than concentrating on either a few cells, as could be obtained by autoradiography, or on average values, such as obtained by biochemical measurements of synchronized populations. The presence of rare cells or minor subpopulations escapes detection. FCM technique allows the measurement through the fluorescence intensity of large number of cells that are labeled with antibodies conjugated to fluorescent reporter molecules to accurately detect the fraction of cells in a population expressing an antigen. Two or more antigens can also be examined simultaneously that permits the correlation of antigen levels with cell cycle. FCM has both diagnostic (leukemia and lymphoma) and prognostic (receptor expression) roles.
Flow cytometry (FCM) is the most practiced member of a family of technologies known variously as automated, analytical and quantitative cytology. Flow cytometry DNA content analysis of human neoplasia provides quantitative information on DNA ploidy, clonal DNA heterogeneity and proliferative activity. The wide use of FCM also stems from the fact that this methodology provides a mean to study the properties of large cell populations rather than concentrating on either a few cells, as could be obtained by autoradiography, or on average values, such as obtained by biochemical measurements of synchronized populations. The presence of rare cells or minor subpopulations escapes detection. FCM technique allows the measurement through the fluorescence intensity of large number of cells that are labeled with antibodies conjugated to fluorescent reporter molecules to accurately detect the fraction of cells in a population expressing an antigen. Two or more antigens can also be examined simultaneously that permits the correlation of antigen levels with cell cycle. FCM has both diagnostic (leukemia and lymphoma) and prognostic (receptor expression) roles.
Über den Autor
Safinaz Ebrahim El-Toukhy, graduated from faculty of science- ain shams university- biochemistry department. Awarded [...] and PhD at the same place. member in many professional associations. attended and organized workshops in prognostic and diagnostic techniques such as comet assay and flow cytometry. Interested in the field of early diagnosis.
Details
Erscheinungsjahr: 2025
Fachbereich: Biophysik
Genre: Biologie, Importe
Rubrik: Naturwissenschaften & Technik
Medium: Taschenbuch
ISBN-13: 9786208457327
ISBN-10: 6208457327
Sprache: Englisch
Einband: Kartoniert / Broschiert
Autor: El-Toukhy, Safinaz
Hersteller: LAP LAMBERT Academic Publishing
Verantwortliche Person für die EU: SIA OmniScriptum Publishing, Brivibas Gatve 197, ?-1039 Riga, customerservice@vdm-vsg.de
Maße: 220 x 150 x 5 mm
Von/Mit: Safinaz El-Toukhy
Erscheinungsdatum: 02.09.2025
Gewicht: 0,119 kg
Artikel-ID: 133964984

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