Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Flower development in Arabidopsis.- Flower development in the Solanaceae.- The ABC of flower development in monocots: the model of rice spikelet.- Model species to investigate the origin of flowers.- Hormones and flower development in Arabidopsis.- Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors.- Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development.- Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and rice.- Isolation of meiocytes and cytological analyses of male meiotic chromosomes in soybean, lettuce and maize.- Genetic and phenotypic analyses of carpel development in Arabidopsis.- Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis.- Floral induction systems for the study of Arabidopsis flower development.- Protoplasting and Fluorescence-activated cell sorting of the shoot apical meristem cell types.- Protoplast Isolation for Plant Single-Cell RNA-seq.- Plant Nuclei Isolation for Single-nucleus RNA Sequencing.- Isolation of nuclei tagged in specific cell types (INTACT) in Arabidopsis.- RNA in situ hybridization on plant tissue sections: expression analysis at cellular resolution.- The GUS reporter system in flower development studies.- Expression and functional studies of leaf, floral and fruit developmental genes in non-model species.- Gene expression analysis by quantitative real-time PCR for floral tissues.- Misexpression approaches for the manipulation of flower development.- Genomic approaches for the study of flower development in floriculture crops.- Multi-omics methods applied to flower development.- Peptidomics methods applied to the study of flower development.- Quantifying gene expression domains in plant shoot apical meristems.- A NanoLuc-based transactivation assay in plants.- A hands-on guide to generate spatial gene expression profiles by integrating scRNA-seq and 3D reconstructed microscope-based plant structures.