Claudio Ortolani: Flow Cytometry Today

Cover: 9783031108358 | Flow Cytometry Today | Claudio Ortolani | Taschenbuch | Paperback

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Beschreibung

This book covers all the technical aspects of flow cytometry needed to set-up the instrument, solve problems encountered in daily work, or necessary for exam preparation. It provides the reader with an in-depth look at the device and its applications.

Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process.

This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers.

Über den Autor

Dr. Claudio Ortolani was a senior consultant (now retired) in Clinical Pathology at the Hospital Dell'Angelo, Venice, Italy.

Zusammenfassung

Explains the technical details of a flow cytometer

Prepares the reader for solving potential problems in daily laboratory work

Covers all aspects of flow cytometric measurements

Inhaltsverzeichnis

Chapter 1. General Principles.- Chapter 2. Signals - Scattering.- Chapter 3. Signals - Fluorescence, Phosphorescence, Impedance, Extinction.- Chapter 4. Fluidics.- Chapter 5. Light Sources.- Chapter 6. Optical Benches.- Chapter 7. Detectors and Electronics.- Chapter 8. Signal Analysis.- Chapter 9. The Cytometric File.- Chapter 10. Data Transformation.- Chapter 11. Data Representation.- Chapter 12. Data Analysis.- Chapter 13. Standards, Setup, Calibration, and Control Techniques.- Chapter 14. Fluorochromes - Overview.- Chapter 15. Fluorochromes Suitable for Antibody Conjugation.- Chapter 16. Fluorochromes That Bind Nucleic Acids.- Chapter 17. Fluorochromes for the Study of the Cell Features.- Chapter 18. Fluorescent Proteins.- Chapter 19. Spillover and Compensation.- Chapter 20. Artifacts.- Chapter 21. Cell Sorting.- Chapter 22. Non-Conventional Flow Cytometry.- Chapter 23. Statistics: A Cytometric Point of View.

Erscheinungsjahr:	2022
Fachbereich:	Allgemeines
Genre:	Biologie
Rubrik:	Naturwissenschaften & Technik
Thema:	Lexika
Medium:	Taschenbuch
Inhalt:	xxix 544 S. 1 s/w Illustr. 544 p. 1 illus.
ISBN-13:	9783031108358
ISBN-10:	3031108353
Sprache:	Englisch
Ausstattung / Beilage:	Paperback
Einband:	Kartoniert / Broschiert
Autor:	Ortolani, Claudio
Auflage:	1st ed. 2022
Hersteller:	Springer Nature Switzerland Springer International Publishing Springer International Publishing AG
Maße:	235 x 155 x 29 mm
Von/Mit:	Claudio Ortolani
Erscheinungsdatum:	17.11.2022
Gewicht:	0,966 kg

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