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Beschreibung
This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers.
These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.
This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers.
These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.
Inhaltsverzeichnis
  • 1.: Techniques for transformation of [...]

  • 2.: Lambda replacement vectors in the construction of genomic DNA libraries

  • 3.: Procedures for cDNA cloning

  • 4.: Making probes

  • 5.: Using cloned DNAs to analyse gene organization and expression

  • 6.: Choices of single-stranded vectors and their application for in vitro mutagenesis

  • 7.: PCR and its applications

  • 8.: DNA sequencing

Details
Erscheinungsjahr: 2002
Fachbereich: Allgemeines
Genre: Biologie, Importe
Rubrik: Naturwissenschaften & Technik
Medium: Taschenbuch
Inhalt: Kartoniert / Broschiert
ISBN-13: 9780199634767
ISBN-10: 0199634769
Sprache: Englisch
Einband: Kartoniert / Broschiert
Autor: Glover, Hames
Glover, D. M.
Hames, David
Redaktion: Glover, D. M.
Hames, B. D.
Auflage: Second
Hersteller: OUP Oxford
Verantwortliche Person für die EU: Libri GmbH, Europaallee 1, D-36244 Bad Hersfeld, gpsr@libri.de
Maße: 234 x 156 x 16 mm
Von/Mit: Hames Glover (u. a.)
Erscheinungsdatum: 01.03.2002
Gewicht: 0,442 kg
Artikel-ID: 108637701