DNA and RNA Isolation Techniques for Non-E...

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Sprache: Englisch

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Beschreibung

This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.

Über den Autor

Dr. Akash Gautam is an Assistant Professor at the Center for Neural and Cognitive Sciences, University of Hyderabad. Dr. Gautam is currently serving as a section editor for the Encyclopedia of Animal Cognition and Behavior (Springer Nature c. 2020).

Zusammenfassung

Accessible guide for junior researchers and those in adjacent fields beginning interdisciplinary research

Presents basic concepts, step-by-step instructions, and abundant illustrations

Addresses the benefits and limitations of each technique, and troubleshoots common issues

Inhaltsverzeichnis

1-Basic Idea About Nucleic Acids For Non-Experts.- 2-General Steps During Isolation Of DNA And RNA.- 3-Phenol-Chloroform DNA Isolation Method.- 4-RNA Isolation By The Guanidinium-Acid-Phenol Method.- 5-Spin Column-Based Isolation Of Nucleic Acid.- 6-Isolation Of Plasmid DNA By Alkaline Lysis.- 7-DNA Isolation By Hydrophilic Ionic Liquid Treatment.- 8-Lithium Chloride Based Isolation Of DNA And RNA.- 9-Isolation Of Bacteriophage DNA By Peg Method.- 10-DNA Isolation By Chelex Method.- 11-DNA Isolation By Lysozyme And Proteinase K.- 12-Salting-Out Method For Blood Samples.- 13-CTAB Or SDA Based Isolation Of Plant's DNA.- 14-DNA Extraction By Spooling Method.- 15-Magnetic Bead-Based Nucleic Acid Isolation.- 16-Density Gradient-Based Nucleic Acid Isolation.- 17-DNA Extraction From Agarose Gel Though Paper-Strips.- 18-Transformation Or Genetic Modification Of Cells/Organisms.- 19-Gene Cloning And Vectors.- 20-Polymerase Chain Reaction (PCR).- 21-Southern And Northern Blotting.- 22-Genome Mapping.- 23-Applications Of DNA Sequencing Technologies For Current Research.

Details

Erscheinungsjahr:	2023
Fachbereich:	Biophysik
Genre:	Biologie , Mathematik , Medizin , Naturwissenschaften , Technik
Rubrik:	Naturwissenschaften & Technik
Medium:	Taschenbuch
Inhalt:	xvi 203 S. 10 s/w Illustr. 48 farbige Illustr. 203 p. 58 illus. 48 illus. in color.
ISBN-13:	9783030942328
ISBN-10:	3030942325
Sprache:	Englisch
Einband:	Kartoniert / Broschiert
Autor:	Gautam, Akash
Auflage:	1st edition 2022
Hersteller:	Springer Nature Switzerland Springer International Publishing
Verantwortliche Person für die EU:	Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com
Maße:	235 x 155 x 13 mm
Von/Mit:	Akash Gautam
Erscheinungsdatum:	31.03.2023
Gewicht:	0,341 kg