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Beschreibung
Mycotoxins - toxic secondary metabolites produced by mycotoxigenic fungi - pose a significant risk to the food chain. Indeed, they may be the most hazardous of all food contaminants in terms of chronic toxicity and legislative limits on their levels in food and feed continue to be developed worldwide. Rapid and reliable methods for the determination of both mycotoxigenic fungi and mycotoxins in food and feed are therefore essential. This book reviews current and emerging methods in this area.

Part one focuses on the essentials of mycotoxin determination, covering sampling, sample preparation and clean-up and key determination techniques, such as chromatographic separation, liquid chromatography-mass spectrometry and immunochemical methods. Part two then goes on to describe quality assurance, official methods and performance criteria for determining mycotoxins in food and feed. Topics covered include laboratory accreditation, method validation and measurement uncertainty. The development and analysis of biomarkers for mycotoxins are discussed in part three. Individual chapters focus on detecting exposure in humans and animals. Part four is concerned with the processes involved in determining mycotoxigenic fungi in food and feed. It also describes the identification of genes and gene clusters involved in mycotoxin synthesis, as well as DNA barcoding of toxigenic fungi. Finally, part five explores some of the emerging methods for mycotoxin analysis, ranging from bio-sensing to spectroscopic techniques.

With its distinguished editor and international team of contributors, Determining mycotoxins and mycotoxigenic fungi in food and feed is a standard reference for all those concerned with reducing mycotoxin contamination in the food chain.
Mycotoxins - toxic secondary metabolites produced by mycotoxigenic fungi - pose a significant risk to the food chain. Indeed, they may be the most hazardous of all food contaminants in terms of chronic toxicity and legislative limits on their levels in food and feed continue to be developed worldwide. Rapid and reliable methods for the determination of both mycotoxigenic fungi and mycotoxins in food and feed are therefore essential. This book reviews current and emerging methods in this area.

Part one focuses on the essentials of mycotoxin determination, covering sampling, sample preparation and clean-up and key determination techniques, such as chromatographic separation, liquid chromatography-mass spectrometry and immunochemical methods. Part two then goes on to describe quality assurance, official methods and performance criteria for determining mycotoxins in food and feed. Topics covered include laboratory accreditation, method validation and measurement uncertainty. The development and analysis of biomarkers for mycotoxins are discussed in part three. Individual chapters focus on detecting exposure in humans and animals. Part four is concerned with the processes involved in determining mycotoxigenic fungi in food and feed. It also describes the identification of genes and gene clusters involved in mycotoxin synthesis, as well as DNA barcoding of toxigenic fungi. Finally, part five explores some of the emerging methods for mycotoxin analysis, ranging from bio-sensing to spectroscopic techniques.

With its distinguished editor and international team of contributors, Determining mycotoxins and mycotoxigenic fungi in food and feed is a standard reference for all those concerned with reducing mycotoxin contamination in the food chain.
Zusammenfassung
Professor Sarah De Saeger is based in the Faculty of Pharmaceutical Sciences at Ghent University, Belgium. Her current research interests include the development of innovative screening and confirmatory mycotoxin analytical methods, masked mycotoxins and sick building syndrome.
Inhaltsverzeichnis
Contributor contact details

Woodhead Publishing Series in Food Science, Technology and Nutrition

Preface

Part I: Determining mycotoxins in food and feed

Chapter 1: Sampling strategies to control mycotoxins

Abstract:

1.1 Food safety and the requirements for international food trade

1.2 Principles of food and feed sampling for mycotoxin analysis

1.3 International guidance on sampling food and feed for mycotoxin analysis

1.4 Uncertainty estimation and designing sound sampling plans for mycotoxin analysis in food and feed

1.5 Quality assurance and quality control procedures in sampling and arrival of the samples at the analytical laboratory

1.6 Strengthening national food control systems

Chapter 2: Sample preparation and clean up in mycotoxin analysis: principles, applications and recent developments

Abstract:

2.1 Introduction

2.2 Methods used for extraction and clean up of mycotoxins from complex matrices

2.3 Recent developments

2.4 Conclusions

2.5 Acknowledgements

Chapter 3: Chromatographic separation techniques for determination of mycotoxins in food and feed

Abstract:

3.1 Introduction

3.2 Thin-layer chromatography in mycotoxin analysis of food and feed

3.3 Gas chromatography in mycotoxin analysis of food and feed

3.4 High-performance liquid chromatography in mycotoxin analysis of food and feed

3.5 Electrophoretic separations in mycotoxin analysis of food and feed

3.6 Future trends

Chapter 4: Mass spectrometry in multi-mycotoxin and fungal spore analysis

Abstract:

4.1 Introduction

4.2 Liquid chromatography-mass spectrometry (LC-MS) methods in multi-mycotoxin analysis

4.3 Liquid chromatographic aspects of multi-mycotoxin methods

4.4 Mass spectrometric aspects of multi-mycotoxin methods

4.5 LC-MS aspects of multi-mycotoxin analysis

4.6 Future trends in liquid chromatography-mass spectrometry analysis

4.7 Conclusions

4.8 Acknowledgements

Chapter 5: Immunochemical methods for rapid mycotoxin detection in food and feed

Abstract:

5.1 Introduction

5.2 Antibody production and characterization

5.3 Specificity of immunochemical methods for rapid mycotoxin detection in food and feed

5.4 Microtiter plate enzyme-linked immunosorbent assay for rapid mycotoxin detection in food and feed

5.5 Non-instrumental rapid tests for mycotoxin detection in food and feed

5.6 Conclusions and future trends

Part II: Quality assurance and official methods for determining mycotoxins in food and feed

Chapter 6: Official methods and performance criteria for determining mycotoxins in food and feed

Abstract:

6.1 Introduction

6.2 Official control laboratories for determining mycotoxins in food and feed

6.3 Establishment of method performance criteria for determining mycotoxins in food and feed

6.4 Official methods for determining mycotoxins in food and feed

6.5 Literature publications of mycotoxin validation studies

6.6 Enforcement of mycotoxin regulations

6.7 Confirmation of results

6.8 Conclusions and future trends

Chapter 7: Ensuring the quality of results from food control laboratories: laboratory accreditation, method validation and measurement uncertainty

Abstract:

7.1 Introduction: why accreditation can be important for laboratories

7.2 Laboratory accreditation and ISO 17025

7.3 Statistical method validation approach for ensuring the quality of results from food control laboratories

7.4 Comparison of a routine method with a reference method for validating the results from food and feed control laboratories

7.5 Measurement uncertainty in the results from food and feed control laboratories

7.6 Conclusions and future trends<

Details
Erscheinungsjahr: 2016
Genre: Ernährungswissenschaft, Importe
Rubrik: Essen & Trinken
Thema: Lebensmittelkunde
Medium: Taschenbuch
Inhalt: Einband - flex.(Paperback)
ISBN-13: 9780081014943
ISBN-10: 0081014945
Sprache: Englisch
Einband: Kartoniert / Broschiert
Redaktion: de Saegar, Sarah
de Saeger, S.
Hersteller: Elsevier Inc
Verantwortliche Person für die EU: Elsevier B.V., Radarweg 29, ?-1043 NX Amsterdam, productsafety@elsevier.com
Maße: 234 x 156 x 23 mm
Von/Mit: Sarah de Saegar (u. a.)
Erscheinungsdatum: 19.08.2016
Gewicht: 0,63 kg
Artikel-ID: 126704613