Agnelo Furtado: Cereal Genomics | Buch

Um unseren Shop nutzen zu können, müssen Sie Javascript in Ihrem Browser aktivieren.

Cover: 9781627037143 | Cereal Genomics | Methods and Protocols | Agnelo Furtado (u. a.) | X

Dekorationsartikel gehören nicht zum Leistungsumfang.

Sprache: Englisch

106,99 €*

inkl. MwSt.

Versandkostenfrei per Post / DHL

Aktuell nicht verfügbar

Sie können kein Produkt in den Warenkorb legen, da Javascript und technisch notwendige Cookies aktiviert sein müssen.

Kategorien:

Der Artikel ist nicht in deutscher Sprache verfasst.
Die Sprache der Produktbeschreibung und Details kann in Englisch angegeben sein. Sollten Sie Verständnisprobleme haben, wenden Sie sich gerne an uns.

Beschreibung

¿In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes. Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described. Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.

Zusammenfassung

Is a comprehensive resource for studying cereal genomes

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: [...]

Inhaltsverzeichnis

DNA Extraction from Vegetative Tissue for Next Generation Sequencing .- DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples) .- RNA Extraction from Cereal Vegetative Tissue.- RNA Extraction from Developing or Mature Wheat Seeds.- cDNA Library Preparation.- Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants.- The Polymerase Chain Reaction (PCR) - General Methods.- Mutation and Mutation Screening.- The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression.- Cloning of DNA Fragments: Ligation Reactions in Agarose Gel.- Rapid Cloning of Genes and Promoters for Functional Analyses.- Genome Walking.- Functional Analysis by Protein Biochemistry.- Genomic Southern Blot analysis.- Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs.- Protein Blotting-Protocol for Beginners.- Genetic Transformation of Wheat via Particle Bombardment.- Sorghum Genetic Transformation by Particle Bombardment.- Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery.- A Protocol for High-throughput Agrobacterium-mediated Barley Transformation.- Agrobacterium-mediated Transformation- Rice Transformation.- Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos.- A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops.- A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data.

Details

Erscheinungsjahr:	2013
Fachbereich:	Botanik
Genre:	Biologie
Rubrik:	Naturwissenschaften & Technik
Medium:	Buch
Seiten:	312
Reihe:	Methods in Molecular Biology
Inhalt:	x 300 S. 10 s/w Illustr. 29 farbige Illustr. 300 p. 39 illus. 29 illus. in color.
ISBN-13:	9781627037143
ISBN-10:	1627037144
Sprache:	Englisch
Ausstattung / Beilage:	HC gerader Rücken kaschiert
Einband:	Gebunden
Redaktion:	Furtado, Agnelo Henry, Robert J.
Herausgeber:	Robert J Henry/Agnelo Furtado
Auflage:	2014
Hersteller:	Humana Press Humana Press Inc. Methods in Molecular Biology
Maße:	260 x 183 x 21 mm
Von/Mit:	Agnelo Furtado (u. a.)
Erscheinungsdatum:	16.11.2013
Gewicht:	0,787 kg

preigu-id: 105718141