Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: [...]

Cancer Biology.- Principles of Cancer Cell Culture.- Storage of Cell Lines.- Characterization and Authentication of Cancer Cell Lines: An Overview.- Online Verification of Human Cell Line Identity by STR DNA Typing.- Cytogenetic Analysis of Cancer Cell Lines.- Cell Culture Contamination.- Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction.- Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics.- Quality Assurance and Good Laboratory Practice.- Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning.- Isolation and Culture of Colon Cancer Cells and Cell Lines.- Isolation and Culture of Melanoma and Naevus Cells and Cell Lines.- Isolation and Culture of Squamous Cell Carcinomalines.- Isolation and Culture of Ovarian Cancer Cells and Cell Lines.- Establishment and Culture of Leukemia-Lymphoma Cell Lines.- Isolation of Inflammatory Cells from Human Tumors.- Isolation of Endothelial Cells from Human Tumors.- Cellular Chemosensitivity Assays: An Overview.- Cell Sensitivity Assays: The MTT Assay.- Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay.- The Differential Staining Cytotoxicity (DiSC) Assay: A Review.- Real-time Cytotoxicity Assays.- FPurification of Annexin V and its use in the Detection of Apoptotic Cells.- Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay.- Molecular Breakpoint Analysis of Chromosome Translocations in Cancer Cell Lines by Long Distance Inverse-PCR.- Cell Migration and Invasion Assays.- Angiogenesis Assays.- Flow Cytometric DNA Analysis of Human Cancers and Cell Lines.- Expression Analysis of Homeobox Genes in Leukemia/Lymphoma Cell Lines.- Measuring Gene Expression from Cell Cultures by Quantitative Reverse-Transciptase Polymerase Chain Reaction.- Proteomic Evaluation of Cancer Cells - Identification of Cell Surface Proteins.- Development of Rituximab-resistant B-NHL Clones:An In Vitro Model for Studying Tumor Resistance to Monoclonal Antibody-mediated Immunotherapy.- High Throughput Screening of Natural Products for Cancer Therapy: A Review.- Analysis of Drug Interactions.- Transfection and DNA-mediated Gene Transfer.- Drug Design and Testing: Profiling of Anti-proliferative Agents for Cancer Therapy Using a Cell-based Methyl-[3H]-Thymidine Incorporation Assay.- Feeder Layers: Co-culture with Non-neoplastic Cells.- Xenotransplantation of Breast Cancers