Combines brand new protocols with heavily revised and improved methodologies

Features lab-ready procedures with step-by-step instruction

Includes expert tips and key implementation advice to ensure successful results

Includes supplementary material: [...]

Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations.- Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2.- Confocal Microscopy: Theory and Applications for Cellular Signaling.- Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer.- Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR®).- Ratiometric [Ca2+]i Measurements in Adherent Cell-lines Using the NOVOstar Microplate Reader.- Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones.- Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells.- Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol Labeling Protocols in Permeabilized Cells.- Single Cell Imaging Techniques for the Real-Time Detection of IP3 in Live Cells.- Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay.- Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry.- Calcium Measurements from Whole Heart Using Rhod-2.- Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteries.- Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration.- Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations.- Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes.- Chimeric G-Proteins in Fluorimetric Calcium Assays: Experience with Opioid Receptors.- Compartmentalizing Genetically-Encoded Calcium Sensors.- Measurement of Cytosolic-Free Ca2+ in Plant Tissue.- Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1).