Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Culture, Expansion, and Differentiation of Human Bone Marrow Stromal Cells.- Differentiation and Phenotyping of Murine Osteoclasts from Bone Marrow Progenitors, Monocytes, and Dendritic Cells.- Culture, Expansion, and Differentiation of Mouse Bone-derived Mesenchymal Stromal Cells.- Feeder-Free Differentiation Assay for Mouse Hematopoietic Stem and Progenitor Cells.- In Vitro Analysis of Energy Metabolism in Bone-marrow Mesenchymal Stromal Cells.- Flow Cytometry Analysis of Mouse Hematopoietic Stem and Multipotent Progenitor Cells.- Flow Cytometry-Based Analysis of the Mouse Bone Marrow Stromal and Perivascular Compartment.- Immunophenotyping of the Medullary B cell Compartment in Mouse Models.- Metabolic Analysis of Mouse Haematopoietic Stem and Progenitor Cells.- Multicolor Immunofluorescence Staining Of Paraffin-Embedded Human Bone Marrow Sections.- 3D microscopy of Murine Bone Marrow Hematopoietic Tissues.- Ex Vivo Whole-Mount Imaging of Leukocyte Migration to the Bone Marrow.- Intrafemoral Delivery of Hematopoietic Progenitors.- Imaging of Bone Marrow Plasma Cells and of their Niches.- Intravital Imaging of Bone Marrow Microenvironment in the Mouse Calvaria and Tibia.- Intravital Imaging of Bone Marrow Niches.- Modeling Human Fetal Hematopoiesis in Humanized Mice.- Engraftment of Human Hematopoietic Cells in Biomaterials Implanted In Immunodeficient Mouse Models.- 3D Engineering of Human Hematopoietic Niches in Perfusion Bioreactor.- Manufacturing a Bone Marrow-on-a-Chip Using Maskless Photolithography.- In Vivo Tracking of Hematopoietic Stem and Progenitor Cell Ontogeny by Cellular Barcoding.- Single Cell Analysis of Haematopoietic Stem Cells.