Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Status of Immune-oncology: Challenges and Opportunities.- Immunological Targets for Immunotherapy: Inhibitory T Cell Receptors.- Measuring Tumor Mutational Burden Using Whole Exome Sequencing.- Germline Genetics in Immuno-Oncology: From Genome-wide to Targeted Biomarker Strategies.- Detection of Microsatellite Instability Biomarkers via Next-generation Sequencing.- Genetic Instability Markers in Cancer.- Validation of Circulating Tumor DNA Assays for Detection of Metastatic Melanoma.- Techniques Associated with Exosome Isolation for Biomarker Development - Liquid Biopsies for Ovarian Cancer Detection.- Identifying MicroRNA Pathway Variants as Biomarkers of Patient Selection for Immune Therapy.- Histone Modifications as Biomarkers for Immunotherapy.- Functional Genome Profiling to Understand Cancer Immune Responsiveness.- Using RNA-sequencing to Characterize the Tumor Microenvironment.- Development of Gene Expression Based Biomarkers on the nCounter® Platform for Immuno-Oncology Applications.- The CRISPR System and Cancer Immunotherapy Biomarkers.- HLA Class I Antigen Processing Machinery Defects in Cancer Cells - Frequency, Functional Significance and Clinical Relevance with Special Emphasis on their Role in T cell-based Immunotherapy of Malignant Disease.- High-parameter Immune Profiling with CyTOF.- The Adaptome as Biomarker for Assessing Cancer Immunity and Immunotherapy.- Single Molecule Arrays for Ultrasensitive Detection of Blood-based Biomarkers for Immunotherapy.- Single-cell Multiplexed Proteomics on the IsoLight Resolves Cellular Functional Heterogeneity to Reveal Clinical Responses of Cancer Patients to Immunotherapies.- Methods to Detect Immunogenic Cell Death in vivo.- Overview of Tissue Imaging Methods.- Multiplex Immunofluorescence Assays.- Multiplexed Immunohistochemical Consecutive Staining on Single Slide (MICSSS): Multiplexed Chromogenic IHC Assay for High-Dimensional Tissue Analysis.- Cyclic Multiplexed-Immunofluorescence (cmIF),A Highly Multiplexed Method for Single-cell Analysis.- High-Plex Spatially Resolved RNA and Protein Detection using Digital Spatial Profiling: A Technology Designed for Immuno-Oncology Biomarker Discovery and Translational Research.- The InSituPlex® Staining Method for Multiplexed Immunofluorescence Cell Phenotyping and Spatial Profiling of Tumor FFPE Samples.- Microbiome as an Immunological Modifier.- Systems Biology for Multi-Platform Data Integration - Overview.- Bioinformatics Tools and Resources for Cancer Immunotherapy Study.- Multiplatform Integrative Analysis of Immunogenomic Data for Biomarker Discovery.- Translating Immuno-Oncology Biomarkers to Diagnostic tests: A regulatory Perspective.