Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Vaccine Design by Reverse Vaccinology and Machine Learning.- Application of Reverse Vaccinology and Immunoinformatic Strategies for the Identification of Vaccine Candidates against Shigella flexneri.- Purification of Prospective Vaccine Antigens from Gram-Positive Pathogens by Immunoprecipitation.- Rapid Surface Shaving for Proteomic Identification of Novel Surface Antigens for Vaccine Development.- Two-Dimensional Electrophoresis Coupled with Western Blot as a Method to Detect Potential Neutralizing Antibody Targets from Gram-Negative Intracellular Bacteria.- Panproteome Analysis of the Human Antibody Response to Bacterial Vaccines and Challenge.- Low-Energy Electron Irradiation (LEEI) for the Generation of Inactivated Bacterial Vaccines.- Design and Production of Hybrid Antigens for Targeting Integral Outer Membrane Proteins in Gram-Negative Bacteria.- Preparation of Trimethyl Chitosan-Based Polyelectrolyte Complexes for Peptide Subunit VaccineDelivery.- Multiepitope Fusion Antigen - MEFA, An Epitope- and Structure-Based Vaccinology Platform for Multivalent Vaccine Development.- Production, Isolation, and Characterization of Bioengineered Bacterial Extracellular Membrane Vesicles Derived from Bacteroides thetaiotaomicron and Their Use in Vaccine Development.- Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures.- Glycine Induction Method: Effective Production of Immunoactive Bacterial Membrane Vesicles with Low Endotoxin Content.- Methods for Assessment of OMV/GMMA Quality and Stability.- Production of Vaccines using Biological Conjugation.- Immunological Assessment of Lung Responses to Inhalational Lipoprotein Vaccines against Bacterial Pathogens.- Determination of Maternal and Infant Immune Responses to Pertussis Vaccination in Pregnancy.- Generation of a Universal Human Complement Source by Large-Scale Depletion of IgG and IgM from Pooled Human Plasma.- Assessment of Serum Bactericidal and Opsonophagocytic Activity of Antibodies to Gonococcal Vaccine Targets.- Opsonophagocytic Killing Assay to Measure Anti-Group A Streptococcus Antibody Functionality in Human Serum.- Neisseria lactamica Controlled Human Infection Model.- Analyzing Macrophage Infection at the Organ Level.- Multi-Color Flow Cytometry and High-Dimensional Data Analysis to Probe Complex Questions in Vaccinology.