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Authoritative and easily accessible, Artificial Riboswitches: Methods and Protocols seeks to serve not only bioengineers who aim to reprogram cell behaviors and molecular biologists who leverage these regulators for genetic studies, but to all researchers interested in this fascinating field.
Authoritative and easily accessible, Artificial Riboswitches: Methods and Protocols seeks to serve not only bioengineers who aim to reprogram cell behaviors and molecular biologists who leverage these regulators for genetic studies, but to all researchers interested in this fascinating field.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: [...]
Computational Design of RNA Libraries for in vitro Selection of Aptamers.- In vitro Selection of RNA Aptamers for a Small-Molecule Dye.- Development of Photoswitchable RNA Aptamer-Ligand Complexes.- Identification of RNA Aptamers Against Recombinant Proteins with a Hexa-Histidine Tag.- Artificial Riboswitch Selection: A FACS-Based Approach.- FRET-Based Optical Assay for Selection of Artificial Riboswitches.- Portable Two-Way Riboswitches: Design and Engineering.- Generation of Orthogonally Selective Bacterial Riboswitches By Targeted Mutagenesis and in vivo Screening.- Dual Genetic Selection of Synthetic Riboswitches in Escherichia coli.- Nucleotide Kinase-Based Selection System for Genetic Switches.- Measuring Riboswitch Activity in vitro and in Artificial Cells with Purified Transcription-Translation Machinery.- Rational Design of Artificial ON-Riboswitches.- Engineering Protein-Responsive mRNA Switch in Mammalian Cells.- Guanine-Tethered Antisense Oligonucleotides as Synthetic Riboregulators.- In Vitro Selection of Allosteric Ribozymes that Sense the Bacterial Second Messenger c-di-GMP.- Dual-Selection for Evolution of in vivo Functional Aptazymes as Riboswitch Parts.- In vivo Screening for Aptazyme-Based Bacterial Riboswitches.- Engineered Riboswitch as Gene-Regulatory Platform for Reducing Antibiotic Resistance.- Construction of Ligand-Responsive microRNAs that Operate Through Inhibition of Drosha Processing.- A Three-Dimensional Design Strategy for a Protein-Responsive shRNA Switch.
| Erscheinungsjahr: | 2014 |
|---|---|
| Fachbereich: | Biophysik |
| Genre: | Biologie |
| Rubrik: | Naturwissenschaften & Technik |
| Medium: | Buch |
| Seiten: | 300 |
| Reihe: | Methods in Molecular Biology |
| Inhalt: |
x
289 S. 30 s/w Illustr. 45 farbige Illustr. 289 p. 75 illus. 45 illus. in color. |
| ISBN-13: | 9781627037549 |
| ISBN-10: | 1627037543 |
| Sprache: | Englisch |
| Herstellernummer: | 86115012 |
| Ausstattung / Beilage: | HC runder Rücken kaschiert |
| Einband: | Gebunden |
| Redaktion: | Ogawa, Atsushi |
| Herausgeber: | Atsushi Ogawa |
| Auflage: | 2014 |
| Hersteller: |
Humana Press
Humana Press Inc. Methods in Molecular Biology |
| Maße: | 260 x 183 x 22 mm |
| Von/Mit: | Atsushi Ogawa |
| Erscheinungsdatum: | 21.02.2014 |
| Gewicht: | 0,765 kg |
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: [...]
Computational Design of RNA Libraries for in vitro Selection of Aptamers.- In vitro Selection of RNA Aptamers for a Small-Molecule Dye.- Development of Photoswitchable RNA Aptamer-Ligand Complexes.- Identification of RNA Aptamers Against Recombinant Proteins with a Hexa-Histidine Tag.- Artificial Riboswitch Selection: A FACS-Based Approach.- FRET-Based Optical Assay for Selection of Artificial Riboswitches.- Portable Two-Way Riboswitches: Design and Engineering.- Generation of Orthogonally Selective Bacterial Riboswitches By Targeted Mutagenesis and in vivo Screening.- Dual Genetic Selection of Synthetic Riboswitches in Escherichia coli.- Nucleotide Kinase-Based Selection System for Genetic Switches.- Measuring Riboswitch Activity in vitro and in Artificial Cells with Purified Transcription-Translation Machinery.- Rational Design of Artificial ON-Riboswitches.- Engineering Protein-Responsive mRNA Switch in Mammalian Cells.- Guanine-Tethered Antisense Oligonucleotides as Synthetic Riboregulators.- In Vitro Selection of Allosteric Ribozymes that Sense the Bacterial Second Messenger c-di-GMP.- Dual-Selection for Evolution of in vivo Functional Aptazymes as Riboswitch Parts.- In vivo Screening for Aptazyme-Based Bacterial Riboswitches.- Engineered Riboswitch as Gene-Regulatory Platform for Reducing Antibiotic Resistance.- Construction of Ligand-Responsive microRNAs that Operate Through Inhibition of Drosha Processing.- A Three-Dimensional Design Strategy for a Protein-Responsive shRNA Switch.
| Erscheinungsjahr: | 2014 |
|---|---|
| Fachbereich: | Biophysik |
| Genre: | Biologie |
| Rubrik: | Naturwissenschaften & Technik |
| Medium: | Buch |
| Seiten: | 300 |
| Reihe: | Methods in Molecular Biology |
| Inhalt: |
x
289 S. 30 s/w Illustr. 45 farbige Illustr. 289 p. 75 illus. 45 illus. in color. |
| ISBN-13: | 9781627037549 |
| ISBN-10: | 1627037543 |
| Sprache: | Englisch |
| Herstellernummer: | 86115012 |
| Ausstattung / Beilage: | HC runder Rücken kaschiert |
| Einband: | Gebunden |
| Redaktion: | Ogawa, Atsushi |
| Herausgeber: | Atsushi Ogawa |
| Auflage: | 2014 |
| Hersteller: |
Humana Press
Humana Press Inc. Methods in Molecular Biology |
| Maße: | 260 x 183 x 22 mm |
| Von/Mit: | Atsushi Ogawa |
| Erscheinungsdatum: | 21.02.2014 |
| Gewicht: | 0,765 kg |
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