Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

1. Design of AAV vectors for Delivery of RNAi.- 2. Design of AAV Vectors for Delivery of Large or Multiple Transgenes.- 3. Ligand Coupling to the AAV Capsid for Cell-Specific Gene Transfer.- 4. Quantitative and Digital Droplet-based AAV Genome Titration.- 5. Single-Stranded DNA Virus Sequencing (SSV-Seq) for Characterization of Residual.- 6. In Situ Hybridization for Detection of AAV-Mediated Gene Expression.- 7. Use of AAV Vectors for CRISPR-Mediated In Vivo Genome Editing in the Retina.- 8. Intravenous Infusion of AAV for Widespread Gene Delivery to the Nervous System.- 9. Intraspinal and Intracortical Delivery of AAV Vectors for Intersectional Circuit Tracing in Non-Transgenic Species.- 10. MRI-Guided Focused Ultrasound for Targeted Delivery of rAAV to the Brain.- 11. AAV-Mediated Gene Delivery to the Spinal Cord by Intrathecal Injection.- 12. Subpial AAV Delivery for Spinal Parenchymal Gene Regulation in Adult Mammals.- 13. Peripheral AAV Injection for Retrograde Transduction of Dorsal Root andTrigeminal Ganglia.- 14. SubILM Injection of AAV for Gene Delivery to the Retina.- 15. Intracameral Delivery of AAV to Corneal Endothelium for Expression of Secretory Proteins.- 16. AAV-Mediated Gene Delivery to the Inner Ear.- 17. Intranasal Delivery of Adenoviral and AAV Vectors for Transduction of the Mammalian Peripheral Olfactory System.- 18. AAV-Mediated Gene Delivery to Taste Cells of the Tongue.- 19. AAV Vectors for Efficient Gene Delivery to Rodent Hearts.- 20. AAV-Mediated Gene Delivery to the Liver: Overview of Current Technologies and Methods.- 21.- AAV-Mediated Gene Delivery to the Lung.- 22. Simplified Purification of AAV and Delivery to the Pancreas by Intraductal Administration.- 23. rAAV-Mediated Gene Delivery to Adipose Tissue.- 24. AAV-Mediated Gene Delivery to the Enteric Nervous System by Intracolonic Injection.